-
Hi Guys, I am using AFM to indent my cells under the physiological conditions (live cells). I am measuring their Young's modulus using recorded force curves. However, I am not sure how can I calculate how deep I am indenting the cells! I was wondering how can I calculate the depth of the indentations. It is worth mentioning that the force I apply
-
Hi Guys, Thank you for your response. I calibrate the tip (ScanAsyst-Fluid) accurately as per manual and also consider the actual tip angle for analysis. Thank you again. Best, Iman
-
Hi Guys, I would like to ask you that is it possible to use ScanAsist-Fluid cantilevers for indentation experiments and force curve measurements instead of DNP cantilevers? If it is possible, how is it going to affect my results? (Young's Modulus). Thank you, Iman
-
Hi Alex, Thank you for your response. In regards to your second answer, I think there is another way of doing it using Nanoscope analysis. I did it like that: I separated the caruves right above the nucleus using red dashed lines in QNM Hsdc-Forcecurve-Image and then exported multiple curves (for example: from 2389-2784). Then I analysed the first curve
-
Hi I am having problem again! This time is with batch processing! I have analyzed just one curve out of ~ 5000 curves. But I donot know how should I extend it to the rest of the curves? Which file should I add to run the history on? When I add the original file it doesnot work! It just gives me one Young's modulus info! Also, if I need the specific
-
And, I also put 0.5 for Poisson's ratio.
-
Hi Guys, Yeah, I figured it after I sent the email. But I am wondering what is the tip half angle for ScanAsyst fluid tip? Is it 15 based on the tip catalog? Thank you for the response. Regards, Iman
-
Hi Alex, Thank you for your response. The problem is when I go to indentation option, after baseline correction, to choose the proper model, when I choose sneddon fit it doesnot show me the modulus. However, when I choose Hertzian it shows me the Young's modulus. How can I fix this? Thank you. Regards, Iman
-
Hi Alex, I will give it a try and will let you know. Thank you. Regards, Iman
-
Dear Alex, For extracting the Young's modulus from Hsdc. files, as you suggested I do the followings: 1) open one representative curve and export it. 2) then open the exported file and correct the baseline using the given option. 3) then select a model (Hertzian) and at this point I am not sure what is next!? I drag the marker pairs over the curve