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Hi, Alex, Thank you very much for your reply. Here are my comments for your suggestions: 1) What type of flow should be employed in the PSI, the same culture medium used? 2) Actually, I've contacted the Team Nanotec for higher tips, but I am not sure if they are able to make a suitable probe as they claim that they have no experience of biological
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Hi, can anyone please give some advice on the following problems of AFM Catalyst scanning and force measurement with the PFQNM mode? 1. How to remove the debris adhered to the tip in situ during the scanning of adhesive samples like cells, without changing the tip? Change of tips would take too much time, especially we have to calibrate the tip again
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Hello, I’ve got several questions concerning AFM catalyst on live cells. Can anyone help me with the following? 1. As we may need to order some probes on demand, what is the typical shape of probes for live cell mechanical measurement, cone or pyramidal? And how shall we set the angle and frequency of probes? 2. How can I export the force and
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What kind of an experimental Category (Scanayst, tapping,contact,….) shall I choose for the following cases? 1) Only the morphology of samples needs imaging? 2) Only the force curves of local regions from biological samples are needed without morphology? Thanks.
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Got it. Thanks, John
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When analysing data of a specified portion of images, is a planefit necessary beforehand? I know it's typical to apply a second- or third-order Flatten or Plane fit to the image before using Roughness analysis if the image shows signs of second- or third-order distortion (e.g., bow due to large scans on large scanners), but I am not sure as the
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1.Do we need to ensure the number of peaks in ETD to be just 1 when estimating tip radius from this height? Actually, I found the tip radius estimated in the absolute method far below the nom value (at least 5 times smaller), after inputting the average deformation into the Height 1 from Apex field. 2. By the way, I've tried both the image roughness
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I see, thanks a lot.
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1. I find the Nanoscope Anaysis (latest version downloaded) invalid in the force curve analysis. Once I open the HSDC file, and click on the QNM-Hsdc Force Curve-Image icon, I get this warning message: The QNMOffline needs either QNMOffline or HaarmoniX or PeakForceQNM Key. Then after I load the image, the interface would show incomplete, the force
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When performing contact mode of AFM on biological samples such as cells, it is very easy for tips adhered and contaminated by cellular debris, how can we clean the tip then instead of replacing it? In chemical or mechanical way?