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How to calculate the depth of the indentations on live cells?

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Iman Jalilian posted on Tue, Feb 4 2014 6:16 PM

Hi Guys,

I am using AFM to indent my cells under the physiological conditions (live cells). I am measuring their Young's modulus using recorded force curves. However, I am not sure how can I calculate how deep I am indenting the cells! 

I was wondering how can I calculate the depth of the indentations. It is worth mentioning that the force I apply to the cells is between 500 pN to 1 nN.

Thank you all,


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Ang Li replied on Wed, Mar 19 2014 9:51 PM

Hi, on the force curve, you have z as the x axis and cantilever deflection d as the y axis, indentation depth is calculated as z-d.   

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