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Hello.I am working with a Bioscope Catalyst with a Nanoscope V Controller.
While imaging in contact mode in air, I am able to to make both a negative (push down) and positive (lift up) Z Move action using the NanoMan Path tab settings.
I have tried to replicate this process in fluids but the system will only let me push downwards on the sample with a negative Z move value. Whenever I try to lift up by entering a positive Z Move value, at any distance starting from only 5nm lift to 150nm lift, the system would give an error message saying that Z limit has been reached and the move in the Z direction failed.
Has anyone ever encountered this problem or knows how to resolve it?Is an upward movement in the Z direction possible using the NanoMan while scanning in fluids?
Thank you !
Usually that sort of thing happens when the Z limit is reduced or if the system is not able to find the surface correctly (it is not really fully engaged.
Hope that helps!
--Bede
Thanks Bede !It seems like the problem was caused by the surface of the sample. There was a layer of contamination on the surface that could have possibly caused some problems with the tip's ability to accurately determine the actual surface. Everything else you mentioned were normal (I had the limit set to maximum by default).I moved on to a cleaner surface and everything worked fine.
Thanks,-Yen