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Correct. Yes, I understand that it might be difficult to get a good force curve in air due to the high adhesion. But you should be able to use a ramp size of up to nearly 20um, if that's what it takes to overcome the adhesion. -Ben
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OK, that's a definite improvement. But I will caution again that the deflection sensitivity in air varies from the deflection sensitivity in fluid. Since this enters into the spring constant calibration as squared term, any error in deflection sensitivity becomes a bigger error in spring constant. -Ben
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You should definitely calibrate both the deflection sensitivity and the thermal tune in the same conditions (air or liquid). The deflection sensitivity does vary somewhat from one to the other. I'm guessing that you see a huge adhesion in air and you are trying to avoid that by calibrating the deflection sensitivity in liquid. But then in liquid
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Sorry, the dsub-9 connector on the signal access module does not provide digital access to the data and control signals. On our older controller architecture there was a provision for basic serial communication through the main 37-pin connector interface. These lines (just TXD, RXD and ground) were broken out and connected to a dsub-9 connector on the
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Sure, this is possible. The only real issue is that the high adhesion between a tipless cantilever and a clean, hard surface can make it difficult to measure a good force curve for deflection sensitivity calibration. I addressed this issue in air for some work I published several years back (B. Ohler, Rev. Sci. Instrum. 78, 063701, 2007). I used the
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Thanks Andres Here's a link to the specific page with more information: http://www2.imec.be/be_en/collaboration/services/cams/calibration-and-resolution-qualifi.html -Ben
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Thanks for reporting this bug. We've investigated it and found the problem. We'll be releasing a new version with the fix soon. Until then, there's a simple workaround. After you flatten the image, be sure to save it before using crop and split. You can either save over the original file or save under a new filename. Then reload the saved
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Current measurements are not generally done under liquids with AFM. Normally the leakage current through the liquid is too large (i.e. larger than the current you're trying to measure). There are groups in the world working to develop insulated AFM probes where only the very apex is exposed that might enable these kinds of measurements. You can
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Fei, The general IO outputs (via the front panel) are bandwidth limited because the signals get sampled and then written back out. This is a different signal path from HSDC. If you sample deflection directly from HSDC (selecting it as a data type) then it has the bandwidth that Bede describes. -Ben
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Fei, The first thing to check is that the data scaling is correct. I would recommend collecting a normal force curve with HSDC and compute the deflection sensitivity yourself using the same data path and file import method that you're using for the thermal tune data. Any errors there will have a big effect. The other thing to check is your calculation