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Hi Charles, If you are using a BioScope II or Catalyst system, it does have the ability to use the thermal tune method for calibration of spring constant. If I recall correctly the BioScope SZ system may also have thermal tune capabilities with the Nanoscope IV controller - although the thermal tune would not be able to go to as high frequencies as
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Dear Mariam, I would think that a ceramic sample is definitely out of the modulus range of a ScanAsyst Air probe. There are other probes available that are better suited for higher modulus samples. However, I am still not sure how well they will do with a surface roughness of ~2 microns. I will pass on your post to one of my collegues who may be able
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When you open the Point & Shoot window the system should automatically switch from 'Image' to 'Ramp' mode. The ramp and capture button is greyed out when the system is in 'Image' mode. You should be able to verify which mode the system is in by looking at the radial buttons at the top of the Point & Shoot window. Select
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Dear Mariam, A 2 micron surface roughness may be quite difficult to image - especially given the height of a typical AFM probe is ~3 microns. There are some probes that have longer tips but what is your sample that you are trying to image - is it soft or hard? Best, Andrea
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Dear Alessandro, To capture Deflection Error vs Height (Z) Sensor: In Ramp Mode, set Channel 2 to 'Height Sensor' on the y-axis and 'Time' on the x-axis. This will allow you to then set the x-axis in Channel 1 to 'Ch 2: Height Sensor' and the y-axis to 'Deflection Error'. When you swtich to Force Volume, this will give
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Hi Shiyue, For ramping in contact mode there are some differences from what you have written: Once you have switched into ramp mode, the scan feedback is turned off and the probe is immediately retracted to a position such that the distance between the probe and the surface is equal to the specified ramp size in the ramp parameters. This happens before
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Hello Igc, Is it possible that the rippling you are seeing could be a result of noise in the image? This would tend to increase as you increased the number of scan lines. As suggested, have you tried changing your scan parameters (setpoint, gains) to see if this affects the rippling? Also, changing your scan angle will help tell you if the rippling
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Hi Mehdi, The auto-tune should be okay for operating in air (we use the manual tune for fluid imaging). I would verify that the target frequency range in your auto-tune parameters matches the range given for the probe you are using. If not, then sometimes the peak the auto-tune chooses may not be the actual resonance peak. With regards to the probes
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Hi Mehdi, If your AFM signals that it is engaged but then the Z scanner immediately goes to being fully extended, this typically indicates that your AFM has false engaged. The scanner fully extends in an attempt to find the surface. You may need to check your cantilever tuning parameters (resonance peak, drive ampltitude. Are you operating in air or
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Hi Gregory, I hope that you have been able to solve the problems that you have been having with your software since you posted this. In case you are still having some issues (and even if not), you might want to make sure that you are using the latest version of Nanoscope software that is available: v8.15R3sr4. Best, Andrea