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See this new paper on ultrahigh resolution imaging and mechanical mapping of bacteriorhodopsin. http://pubs.acs.org/doi/abs/10.1021/nl202351t. I thought I would post this link as nice example of the science that can be done using the AFMs high resolution imaging power, coupled with the inherent information...
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Nothing like a quality sample preparation (thanks Natalia!) and an awesome AFM to get great soft-sample high-speed images (thanks Shuiqing & Adam!). This new video shows height and phase of freshly prepared Celgard; the images are at ~20um/s or 10Hz scan rate for 1um images. In the video, we also...
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Interesting debate going on linked-in. Reposting here for comment as well. **** Normal 0 false false false EN-US X-NONE X-NONE What qualifies a as a high speed AFM? There has been a lot of discussion on what it takes to claim high speed. Should a baseline criteria be to demonstrate at least an order...
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For those interested in dynamic imaging with the AFM: Attached is a link to a Dimesnion FastScan video of DNA taken in tapping mode in fluid at a rate 1 frame per second. We took this data in collaboration with Y. Lyubchenko, at the University of Nebraska Medical Center, as a proof of concept experiment...
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Today Bruker Nano Surfaces Division announced the innovative and unique Dimension FastScan AFM, which delivers a significant breakthrough in improved imaging speed without sacrificing nanoscale resolution. The Dimension FastScan enables users to obtain usable data significantly faster than is possible...
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Hi Crimsonhen, It is a question of working on the right side of the peak (for noncontact) vs the left side of the peak (for tapping). To be more precise, in what is sometimes referred to as non-contact mode in ambient conditions, the drive frequency is set on the right side of the peak at a location...
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What is the practical upper limit (if any) on temperature for TappingMode with a standard TESPA or RTESPA probe?
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What is the maximum viscosity the tapping mode can perform in? Does scan assist make the imaging easier in high viscosity fluids
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How does PeakForce QNM mode comparing to Tapping mode Phase Imaging and Harmonix mode
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Atomic force microscopy (AFM) provides the ability to perform three-dimensional measurements of surface structures at nanometer-to-subangstrom resolution in ambient and liquid environments. These capabilities have led to ground-breaking life sciences advances in the investigation of DNA, proteins, and...
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The human tooth has two main calcified parts with quite different mechanical properties. The enamel is hard and brittle, while the dentin is tough, and can absorb and distribute stress. Enamel and dentin meet at the dentino-enamel junction (DEJ). What is the nanometer-scale anatomy of tooth dentin, enamel...
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In addition to its high resolution imaging capabilities, atomic force microscopy (AFM) has emerged as a powerful tool for measuring both the nanomechanical properties and interaction forces of biomolecular complexes. While the majority of these types of AFM studies have been conducted on isolated molecules...
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Multi-Modal Imaging and Measurements Correlating Optical and Atomic Force Microscopy
Posted to
Application Notes
by
SeanHand
on Tue, Dec 29 2009
Filed under: MIRO, Cells, TappingMode, Force Curve, OTESPA, BioScope II
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The best research instruments not only acquire the intended data, but actually increase productivity. Bruker’s BioScope Catalyst Atomic Force Microscope (AFM) accelerates innovative research by reducing the time and effort needed to combine the proven techniques of light microscopy with the unique...
Posted to
Brochures & Data Sheets
by
Stephen Minne
on Wed, Dec 16 2009
Filed under: Brochure, MIRO, BioScope, Catalyst, Peak Force Tapping, 8.10, ScanAsyst, PeakForce QNM, TappingMode