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In soft tapping you start with a small free amplitude (Afree) and you use a relatively large setpoint to free amplitude ratio (Asp/Afree as large as possible, but usually greater than about 90%). Note that Afree should be measured within a few um of the sample since the cantilever is damped during the...
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Q: Is there any output in the QNM mode which is comparable to the phase imaging in tapping? What is the difference between Inphase and Quadrature outputs, in layman terms? Why does the quadrarture seem to have more noise? A: The inphase is the Rcos(theta) and quadrature is the Rsin(theta) outputs from...
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See this new Nano Letters paper on ultrahigh resolution imaging and mechanical mapping of bacteriorhodopsin. http://pubs.acs.org/doi/abs/10.1021/nl202351t. I thought I would post this link as nice example of the science that can be done using the AFMs high resolution imaging power, coupled with the inherent...
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PS: A good test of the robustness of a system is to scan Celgard® in different orientations; here is an even faster video (23Hz) where the mesh is perpendicular to the scan. Enjoy, Steve
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I was reading an AFM site and saw a contrived claim trying to make a comparison that was >50% off the benchmark and thought it would be a good topic to open up for discussion. The comment was to the effect of: by scanning at a slower scan speed we are actually going faster because our poor scanner...
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It’s good to see some community attempts at replicating the performance of the Dimension FastScan. The screen shot below is of a 1um, 512x512, 22Hz, Video of Celgard® taken with the Dimension FastScan (Video link at end). This demonstration is over twice as fast as the 10Hz video we show above...
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Nothing like a quality sample preparation (thanks Natalia!) and an awesome AFM to get great soft-sample high-speed images (thanks Shuiqing & Adam!). This new video shows height and phase of freshly prepared Celgard; the images are at ~20um/s (or 10Hz scan rate for 1um images): In the video, we also...
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Bruker Dimension Fastscan video of a 180nm Grating at 11Hz, 30um
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Posted to
Webinars and Video
on Fri, Sep 17 2010
Filed under: TappingMode, Video, Closed-Loop System, Edge, AFM, Atomic Force Microscope, Dimension Edge, 3D characterization, MFM
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The ability of atomic force microscopy (AFM) to create three-dimensional micrographs with nanometer resolution has made it an essential tool in applications ranging from semiconductor processing to biology. In addition to this topographical imaging, the AFM can also probe nanomechanical and other fundamental...
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Normal 0 false false false MicrosoftInternetExplorer4 TappingModeTM imaging has proved to be the most versatile mode of atomic force microscopy (AFM) in ambient conditions where the presence of a fluid layer (condensed water vapor and other contaminants) severely limits the applicability of both, contact...
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Atomic Force Microscopy (AFM) operates by scanning a sharp tip, supported on a sensitive force-sensing cantilever, over the sample and thereby producing a three-dimensional image of the surface. As the tip scans across the samples, changes in the interactions with the surface alter the vertical deflection...
Posted to
Application Notes
by
BrukerApplications
on Mon, Jan 4 2010
Filed under: TappingMode, BioScope II, AFM, Neurodegenerative Diseases, Fluorescence Microscopy, Huntington’s Disease
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Atomic force microscopy (AFM) is used to investigate structural aspects of cataract formation. High-resolution imaging of native lens membranes and the constitutive protein components was achieved using a customized Veeco atomic force microscope.