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Hi, in ideal case, if the sample is purely elastic and the experiment condition follow exactly the contact geometry of Hertzian or Sneddon models, the fitting boundary shouldn't affect the results. but this is not the case for most of the samples and different fitting range would give different results. generally speaking, we would like to include
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Hi, I guess now all the manuals are converted to help file embedded in the software. you can simply open the help html and either browse or search for certain topics. Ang Li
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Hi, it's a well known phenomenon depending on tapping force. you can simply change the setpoint during tapping mode imaging and observe the change (sometimes total inversion) of phase image. it's because the origin of the phase shift is complicated and could be from adhesion and viscoelasticity and under different tapping force, different source
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Hi, in Nanoscope Analysis 1.50, you can find 'calculate S parameters' under roughness analysis and under 'S parms-spatial' you can find 'Sal' which is the Auto-Correlation Length, defined as the measure of the distance over the surface such that the new location will have minimal correlation with the original location. Hope it
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Hi, on the force curve, you have z as the x axis and cantilever deflection d as the y axis, indentation depth is calculated as z-d.
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Hi, Simply set the feedback gain to 0 will be constant height mode.
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Hi, to your last question, if you need accurate force during your creep test, you do need to calibrate the cantilever spring constant and deflection sensitivity that will convert the deflection error in your image to the actual force.
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Hi, Bruker offers an option of upgrading to 64bit Win7 system but it will be charged I guess since it involves some hardware reconfiguration and the new software will be version 9 instead of version 8. Please feel free to consult your local Bruker contact for the quotation. Thanks!
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Hi, Please try to change the parameters before you engage and they should be firstly automatically set upon engage (for a few seconds at the beginning of imaging) and later change back to user preset value during imaging. Please let me know if the procedure doesn't work and I can pass you another procedure to 'lock' the values.
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Hi, Jimmy, Your results could be explained from science point of view that your cells are viscoelastic instead of elastic materials. The major difference between FV mode and QNM mode is the indentation rate, that FV mode is usually 100-1000 time slower. Simply speaking, same force but slower indentation generally gives rise to larger deformation on